منابع مشابه
Chinese Hamster Liver Glutamine Synthetase
Purification procedures to prepare homogeneous glutamine synthetase from Chinese hamster liver and a sensitive radioisotope assay for the enzyme are described. The enzyme has a molecular weight of 335,000 as determined by sedimentation in a glycerol gradient, and consists of 8 identical subunits with molecular weights of 42,000 as determined by mobility in sodium dodecyl sulfate polyacrylamide ...
متن کاملMetabolic compartmentation of vertebrate glutamine synthetase: putative mitochondrial targeting signal in avian liver glutamine synthetase.
The evolution of uricoteley as a mechanism for hepatic ammonia detoxication in vertebrates required targeting of glutamine synthetase (GS) to liver mitochondria in the sauropsid line of descent leading to the squamate reptiles and archosaurs. Previous studies have shown that in birds and crocodilians, sole survivors of the archosaurian line, hepatic GS is translated without a transient, N-termi...
متن کاملChicken Brain Glutamine Synthetase and Comparison with Chicken Liver Mitochondrial Glutamine Synthetase*
Chicken brain glutamine synthetase has been found to be localized in the cytosolic fraction of this tissue in contrast to its mitochondrial location in chicken liver. Despite this difference in subcellular distribution, the enzyme from brain exhibits the same molecular weight during sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has the same isoelectric point, and is immunochemicall...
متن کاملSubcellular location of chicken brain glutamine synthetase and comparison with chicken liver mitochondrial glutamine synthetase.
Chicken brain glutamine synthetase has been found to be localized in the cytosolic fraction of this tissue in contrast to its mitochondrial location in chicken liver. Despite this difference in subcellular distribution, the enzyme from brain exhibits the same molecular weight during sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has the same isoelectric point, and is immunochemicall...
متن کاملGlutamine Synthetase. Iv.its Formation in Rat Liver following Partial Hepatectomy and during Repletion.
The change in glutamine synthetase activity has been studied under two conditions of liver growth, namely, mitotic growth after partial hepatectomy and nonmitotic growth during repletion after a fast. The surgical operation caused the enzyme activity in the liver to diminish to one half its normal value within one week after the operation and to remain at this reduced level for the duration of ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)81106-5